Search results for " mikrobiologi"
showing 10 items of 11 documents
Gut Microbiome Associates With Lipid-Lowering Effect of Rosuvastatin in Vivo
2018
Source at https://doi.org/10.3389/fmicb.2018.00530 . Background: Statin has been widely used to treat hyperlipidemia because of its high potency in decreasing cholesterol levels. The present study aimed to examine the lipid-lowering effect of rosuvastatin and the composition, diversity and species abundance of gut microbiome in association with rosuvastatin efficacy. TRIAL REGISTRATION: ChiCTR-ORC-17013212 at the First Affiliated Hospital of Dalian Medical University, November 2, 2017. Results:Totally 64 patients with hyperlipidemia were treated with 10 mg/day of rosuvastatin for 4-8 weeks. Blood lipid indicators triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), lo…
Dissemination of a Carbapenem-Resistant Acinetobacter baumannii Strain Belonging to International Clone II/Sequence Type 2 and Harboring a Novel AbaR…
2013
ABSTRACT An outbreak of hospital-acquired Acinetobacter baumannii infections, caused by a bla OXA-23 -positive carbapenem-resistant strain belonging to international clone II/ST2, was detected in Latvia. The strain was partially equipped with the armA gene and the intI1-aacA4-catB8-aadA1-qacE Δ 1 class 1 integron. In addition, the strain carried AbaR25, a novel AbaR4-like resistance island of ∼46,500 bp containing structures similar to the previously described AbaR22 and Tn 6167 islands. AbaR25 was characterized by the occurrence of a second copy of Tn 6022a interrupted by Tn 2006 carrying the bla OXA-23 gene.
Slow Infection due to Lowering the Amount of Intact versus Empty Particles Is a Characteristic Feature of Coxsackievirus B5 Dictated by the Structura…
2019
Enterovirus B species typically cause a rapid cytolytic infection leading to efficient release of progeny viruses. However, they are also capable of persistent infections in tissues, which are suggested to contribute to severe chronic states such as myocardial inflammation and type 1 diabetes. In order to understand the factors contributing to differential infection strategies, we constructed a chimera by combining the capsid proteins from fast-cytolysis-causing echovirus 1 (EV1) with nonstructural proteins from coxsackievirus B5 (CVB5), which shows persistent infection in RD cells. The results showed that the chimera behaved similarly to parental EV1, leading to efficient cytolysis in both…
New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers
2018
A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significan…
A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses
2018
AbstractEnteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybr…
Ocena ścieków browarnianych w wybranych punktach linii technologicznej
2016
Źródłem ścieków browarnianych są: warzelnia, fermentownia, leżakownia, rozlewnia oraz miejsce mycia opakowań. W zależności od etapu produkcyjnego ścieki te cechują zróżnicowane parametry mikrobiologiczne i fizykochemiczne. Ze względu na dużą zawartość substancji organicznych mogą one stanowić pożywkę dla różnych grup mikroorganizmów. Celem badań była ocena mikrobiologiczna ścieków browarnianych w trzech różnych punktach linii technologicznej ze szczególnym uwzględnieniem obecności mikroorganizmów potencjalnie chorobotwórczych. Materiał badawczy stanowiły ścieki pobrane z komór zbierających ścieki 1 i 2 oraz kolektora przy wylocie do rzeki. Badania mikrobiologiczne obejmowały oznaczenia ogól…
Biotransformacje pochodnych terpenoidowych przy użyciu cyjanobakterii
2012
Human enterovirus group B viruses rely on vimentin dynamics for efficient processing of viral nonstructural proteins
2020
We report that several viruses from the human enterovirus group B cause massive vimentin rearrangements during lytic infection. Comprehensive studies suggested that viral protein synthesis was triggering the vimentin rearrangements. Blocking the host cell vimentin dynamics with IDPN did not significantly affect the production of progeny viruses and only moderately lowered the synthesis of structural proteins such as VP1. In contrast, the synthesis of the non-structural proteins 2A, 3C, and 3D was drastically lowered. This led to attenuation of the cleavage of the host cell substrates PABP and G3BP1 and reduced caspase activation, thus leading to prolonged cell survival. Furthermore, the loc…
Vesiclepedia:A Compendium for Extracellular Vesicles with Continuous Community Annotation
2012
Vesiclepedia is a community-annotated compendium of molecular data on extracellular vesicles.
Ocena mikrobiologiczna niepasteryzowanych soków owocowych i warzywnych
2017
Celem pracy była ocena parametrów fizykochemicznych oraz mikrobiologicznych niepasteryzowanych soków owocowych i warzywnych podczas próby przechowalniczej. Analizie poddano takie parametry fizykochemiczne, jak: pH, kwasowość ogólną, gęstość względną oraz zawartość ekstraktu. W badaniach mikrobiologicznych określono ilość mikroorganizmów oraz przeprowadzono ocenę jakościową badanych soków. Przetrzymywanie otwartych soków przez jedną godzinę w temperaturze pokojowej nie wpłynęło istotnie na zmianę parametrów fizykochemicznych. W trakcie badań odnotowano wzrost ogólnej liczby drobnoustrojów, stwierdzono obecność bakterii grupy coli oraz bakterii rodzaju Pseudomonas. Nie stwierdzono obecności S…